REGENOLIDE™

LATEST DISCOVERY IN HAIR GROWTH

Stimulating hair follicles to regenerate hair.

LATEST DISCOVERY IN HAIR GROWTH

Augmentation of hair growth factors in cultured primary human hair follicle dermal papilla cells (HFDPCs) treated with REGENOLIDE.

REGENOLIDE is a novel Ashwagandha fraction discovered to stimulate hair growth factors by scientist Franco Cavaleri B.Sc PhD.c

Human hair has multiple functions such as skin protection, preservation of body heat, and sensing and responding to external stimuli. The state of scalp hair can be a factor that influences self-esteem and plays a major role in beauty, heredity, and culture. Even though temporary hair shedding is common in all humans, massive irreversible hair loss or alopecia is often distressing for both men and women and can have a significant impact on psychology and social behavior. Hair follicle dermal papilla cells are specialized cell types of mesenchymal origin that are located at the bottom of hair follicles. These cells control keratinocyte activity and are crucial for hair formation and cycling. In this study, the efficacy of Ashwagandha extract (ASH-Ext2) on hair-induction was studied using primary cultured HFDPC cells. β-Catenin was considered a primary marker as studies have shown that induction of β-Catenin activity or expression in the dermal papilla cells leads to the expression of pluripotent factors and ultimately anagen hair cycle induction and folliculogenesis (Choi, 2020; Li et al., 2011; Zhou et al., 2016). Since ASH-Ext2 has a high Withaferin A content, this extract at higher doses (>2µg/ml) exerted cytotoxicity. Therefore, all experiments were done with 2µg/ml or lower doses. Primary HFDPC cells were treated with ASH-Ext2 at 0.5, 1 and 2 µg/ml for 24 hrs and at 1 µg/ml for 24 and 48 hrs. A concentration-dependent (0.5 µg/ml P=0.0508 and 2 µg/ml P=0.007) (Figure 5A-B) and time-dependent (Figure 5C-D) increase in β Catenin expression were observed in HFDPC cells treated with ASH-Ext2. An increase in β Catenin expression was also observed in HFDPC cells treated with 500 nM Rapamycin (P=0.0189) and 3 µg/ml Withaferin A (P=0.0962) as compared to control (Figure 5E-F). HFDPC cells also showed up-regulation in other markers like telomerase reverse transcriptase (TERT) (P=0.0073) (Figure 5G-H), mTOR3, and Phospho-mTOR3 (Figure 5I). Furthermore, ASH-Ext2 treated HFDPC cells formed a higher number of colonies in soft-agar culture than the untreated group (10.66±2.51 vs 15.33±4.50) (Figure 5J-K).  

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